Description
CD49d (Anti–VLA-α4), clone L25, is derived from the fusion of mouse Sp2/0 cells with popliteal lymph node cells from a BALB/c mouse immunized with a CD8+ T-cell line.
CD49d (Anti–VLA-α4) recognizes the 150-kilodalton (kd) α chain of very-late antigen (VLA)-4, a member of the integrin family of cell adhesion molecules. VLA-4, like other integrins, is a noncovalently associated heterodimeric glycoprotein composed of α and β subunits and is involved in cell–cell and cell–extracellular matrix interactions. The β chain of the VLA-4 complex is the CD29 antigen, a 130-kd glycoprotein. The CD29 antigen, also known as the β1 subunit, is common to the VLA family of integrins. When acting as a matrix receptor, the CD49d antigen binds to CS-1, an alternatively spliced domain of fibronectin. When functioning as a cell receptor, the CD49d antigen binds to the vascular cell-adhesion molecule-1 (VCAM-1). The interaction between the CD49d antigen and VCAM-1 is known to play an important role in stabilizing the adhesion of lymphocytes to endothelial cells and in mediating B-lymphocyte precursor/bone marrow stromal cell adhesion. The CD49d antigen, when associated with the β7 integrin, forms a lymphocyte homing receptor for Peyer"s patch, binding to the mucosal vascular addressin MAdCAM-1. The CD49d antigen is also involved in CD3-dependent CD4+T-lymphocyte activation via its interaction with fibronectin.
Format
- FormatPurified
Other Formats→
- PE
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Preparation and Storage
The monoclonal antibody is supplied as 200 µg purified immunoglobulin in 0.2 mL (1.0 mg/mL) of phosphate-buffered saline (PBS) containing 0.01% sodium azide without gelatin. The PE conjugate is supplied as 1.6 µg in 1.0 mL (1.6 µg/mL) of PBS containing gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
