Description
The TRFK5 antibody reacts with mouse interleukin-5 (IL-5) and cross-reacts with human IL-5. The TRFK5 antibody has been reported to cross react with IL-5 from rhesus monkey. This is a neutralizing antibody.
Format
- FormatPurified
Other Formats→
- APC
- NA/LE
- PE
Suggested Companion Products
Purified Rat Anti-Mouse/Anti-Human IL-5 TRFK5RUO 0.5mgCat No: 554393
Purified NA/LE Hamster Anti-Mouse CD3e 145-2C11RUO 0.5mgCat No: 553057
Purified NA/LE Hamster Anti-Mouse CD28 37.51RUO 0.5mgCat No: 553294
Recombinant Mouse IL-2 RUO 20µgCat No: 550069
Recombinant Mouse IL-4 RUO 10µgCat No: 550067
Protein Transport Inhibitor (Containing Monensin) RUO 0.7mLCat No: 554724
Recombinant Mouse IL-5 RUO 5µgCat No: 554581
Recombinant Human IL-5 RUO 5µgCat No: 554606
Resources & Tools | ||||||
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SpectrumViewer | Download TDS | Regulatory Document Website |
Preparation and Storage
Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species testing during development may have been performed with a different format of the same clone.Selected applications have been tested for cross-reactivity.
Blocking Control for Intracellular Staining: The purified TRFK5 antibody can be used as a blocking control to demonstrate specificity of IL-5 staining by PE~TRFK5 or APC~TRFK5 antibodies (Cat. No. 554395, 554396). To perform this control, the fixed/permabilized cells (~ 1X10^6 cells) can be incubated with 1-10 µg of the Purified Rat Anti-Mouse/Anti-Human IL-5 antibody (Cat. No. 554392) for 20 minutes at 4°C, prior to staining with PE~TRFK5 or APC~TRFK5 antibody.