Description
The 8D4-8 monoclonal antibody reacts with human interleukin-4 (IL-4). The immunogen used to raise the 8D4-8 hybridoma was recombinant human IL-4. The 8D4-8 antibody binds to an epitope that is different than the epitope recognized by the MP4-25D2 antibody (Cat. No. 554485).
Clone 8D4-8 displays an increased amount of non-specific binding to dead cells when compared to the clone MP4-25D2.It is recommended to use a fixable viability dye in conjunction with this clone.
Format
- FormatPurified
Other Formats→
- APC
- Alexa Fluor® 488
- BV421
- PE
- PE-Cy™7
- PerCP-Cy™5.5
- V450
Suggested Companion Products
PE Mouse Anti-Human IL-4 8D4-8RUO 0.1mgCat No: 554516
Purified Mouse Anti-Human IL-4 8D4-8RUO 0.5mgCat No: 554515
Biotin Rat Anti-Human IL-4 MP4-25D2RUO 0.5mgCat No: 554483
Recombinant Human IL-4 RUO 5µgCat No: 554605
Human IL-4 ELISA Set RUO 20Plate(s)Cat No: 555194
Human IL-4 ELISA Kit II RUO 2Plate(s)Cat No: 550614
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Preparation and Storage
Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
1. Blocking Control for Intracellular Staining: The purified 8D4-8 antibody (Cat. No. 556917) can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated-8D4-8 antibodies. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
2. ELISA Capture: The purified 8D4-8 antibody (Cat. No. 554515) is useful as a capture antibody for a sandwich ELISA for measuring human IL-4 protein levels. Purified 8D4-8 antibody can be paired with the biotinylated MP4-25D2 antibody (Cat. No. 554483) as the detecting antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. For testing IL-4 in serum or plasma, the OptEIA™human IL-4 ELISA set (Cat. No. 555194) or OptEIA™ human IL-4 ELISA kit (Cat. No. 550614) are recommended.
