Description
The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The immunogen used to generate the MP2-8F8 hybridoma was COS-expressed recombinant mouse IL-3.
Format
- FormatPurified
Other Formats→
- NA/LE
- PE
Suggested Companion Products
Biotin Rat Anti-Mouse IL-3 MP2-43D11RUO 0.5mgCat No: 554384
Recombinant Mouse IL-3 RUO 10µgCat No: 554579
Mouse IL-3 ELISA Set RUO 20Plate(s)Cat No: 555228
Purified NA/LE Rat Anti-Mouse IL-3 MP2-8F8RUO 0.5mgCat No: 554379
PE Rat Anti-Mouse IL-3 MP2-8F8RUO 0.1mgCat No: 554383
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Preparation and Storage
Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
ELISA: The purified MP2-8F8 antibody (Cat. No. 554381) is useful for measuring mouse IL-3 protein levels when utilized as a capture antibody in sandwich ELISA. Purified MP2-8F8 antibody can be paired with the biotinylated MP2-43D11 antibody (Cat. No. 554384) as the detecting antibody, with recombinant mouse IL-3 (Cat. No. 554579) as the standard. Purified MP2-8F8 antibody should be titrated 2-6 µg/mL to determine the optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse IL-3 (Cat. No. 554579), ranging from ~2000 to 15 pg/mL, are recommended for inclusion in each ELISA plate.When measuring mouse IL-3 in serum or plasma, investigators are encouraged to utilize BD OptEIA™ Mouse IL-3 Set (Cat. No. 555228).
Note: This ELISA pair has been reported to show no cross-reactivity with the following the cytokines:
Mouse IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF
Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23, SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Neutralization:The NA/LE™ MP2-8F8 antibody (Cat. No. 554379) has been reported to be useful for neutralization of mouse IL-3 bioactivity.
Flow cytometric analysis and blocking control for intracellular staining: The MP2-8F8 antibody is useful for flow cytometric analysis in identifying and enumerating IL-3 producing cells within mixed cell populations. PE-conjugated MP2-8F8 antibody (Cat. No. 554383) is especially suitable for these studies.In addition, purified MP2-8F8 antibody can be used as a blocking control to demonstrate specificity of IL-3 staining by PE-conjugated MP2-8F8 antibody. To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1 - 10 µg of unlabeled MP2-8F8 antibody (Cat. No. 554381) for 20 minutes at 4°C, prior to staining with PE-MP2-8F8. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
