Description
Streptavidin is prepared chromatographically from Streptomyces avidinii. When excited by a laser beam at 488 nm, Streptavidin PE emits red-orange light with an emission maximum at approximately 580 nm. The emitted light is collected in the fluorescence 2 (FL2) channel.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Other Formats→
- APC
Resources & Tools | ||||||
---|---|---|---|---|---|---|
Spectrum Viewer | Panel Designer | SpectrumViewer | Download MSDS | Download TDS |
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
- Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline — Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.
- Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.