Description
IgM is an important component in the first line of defense against foreign pathogens, but may also play a role in autoimmune diseases.IgM monomers consist of two light and two heavy chains.Unlike the heavy chain of an IgG antibody which contains 3 constant Ig domains, the µ heavy chain of IgM contains 4 constant Ig domains.Five IgM monomers complex with a small polypeptide (J-chain) to form pentameric IgM that can be found in human plasma.In an immune response, the binding of IgM to a cell surface antigen enables C1q to activate interactions with downstream components in the classical complement pathway.Mature B lymphocytes express IgM.The G20-127 monoclonal antibody binds to the heavy chain of human IgM. The G20-127 antibody is not thought to react with other immunoglobulin heavy chain isotypes.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Other Formats→
- APC
- BB515
- BUV395
- BV421
- BV480
- BV510
- BV605
- BV650
- BV711
- BV786
- Biotin
- FITC
- PE-CF594
- PE-Cy™5
- PerCP-Cy™5.5
- Purified
- V450
Suggested Companion Products
PE Mouse IgG1, κ Isotype Control RUO 100TestsCat No: 555749
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test.We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
