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当前位置: 首页 > 产品中心 > peptide > BD Biosciences/BD FastImmune™ CD4 Intracellular Cytokine Detection Kit Anti-Hu-IL-2/CD69/CD4/CD3/25Tests/337187
商品详细BD Biosciences/BD FastImmune™ CD4 Intracellular Cytokine Detection Kit Anti-Hu-IL-2/CD69/CD4/CD3/25Tests/337187
BD Biosciences/BD FastImmune™ CD4 Intracellular Cytokine Detection Kit Anti-Hu-IL-2/CD69/CD4/CD3/25Tests/337187
BD Biosciences/BD FastImmune™ CD4 Intracellular Cytokine Detection Kit Anti-Hu-IL-2/CD69/CD4/CD3/25Tests/337187
商品编号: 337187
品牌: bdbiosciences
市场价: ¥22437.45
美元价: 12821.40
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product DetailsRecommended AssayReferences

Description

The BD FastImmune™ CD4 intracellular cytokine detection kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD4+ T lymphocytes in whole blood. applications include studies of T-cell responses to antigens, such as herpes viruses, HIV, and tumor antigens.

Contents: Antibody Cocktail (IL-2 FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC), Isotype Ctrl Cocktail, Co-Stimulatory Antibodies (CD28/CD49d), Brefeldin A (BFA) Sol., EDTA Solution, BD FACS™ Lysing Solution (10x), BD FACS Permeabilizing Solution 2 (10x).

Preparation and Storage

Upon receipt, thaw BFA, dispense into 10 µL aliquots, and store at –20°C.

Before use, dilute BD FACS lysing solutionand BD FACS Permeabilizing Solution 21:10 in deionized (DI) water. Use at roomtemperature.

Store vials at 2°C–8°C. Conjugated formsshould not be frozen. Protect from exposureto light. Each reagent is stable untilthe expiration date shown on the bottlelabel when stored as directed. Alterationin the appearance of the reagent, such asprecipitation or discoloration, indicatesinstability or deterioration. In such cases,do not use the reagent.

  1. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. Wayne, PA: Clinical and Laboratory Standards Institute; 1998. NCCLS document H42-A.

  2. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 1991. NCCLS document M29-T2.

  3. Asanuma H, Sharp M, Maecker HT, Maino VC, Arvin AM. Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus determined by intracellular detection of cytokine expression. J Infec Dis. In press. 2000;181:859-866.

  4. He XS, Rehermann B, Lopez-Labrador FX, et al. Quantitative analysis of hepatitis C virus-specific CD8+ T cells in peripheral blood and liver using peptide-MHC tetramers. Proc Natl Acad Sci USA. 1999;96:5692-5697.

  5. Komanduri KV, Viswanathan MN, Wieder ED, et al. Restoration of cytomegalovirus-specific CD4+ T-lymphocyte responses after ganciclovir and highly active antiretroviral therapy in individuals infected with HIV-1. Nat Med. 1998;4:953-956.

  6. Lee PP, Yee C, Savage PA, et al. Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients. Nat Ned. 1999;5:677-685.

  7. Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression. Cytometry. 1998;34:207-215.

  8. Maino VC, Suni MA, Wormsley SB, Carlo DJ, Wallace MR, Moss RB. Enhancement of HIV-1 antigen-specific CD4+ T cell memory in subjects with chronic HIV-1 infection receiving an HIV-1 immunogen. AIDS Res Hum Retroviruses. In press.

  9. Maino VC. Rapid assessment of antigen induced cytokine expression in memory T cells by flow cytometry. Vet Immunol Immunopathol. 1998;63:199-207.

  10. Mehta BA, Maino VC. Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4+ T lymphocytes by flow cytometry. J Immunol Methods. 1997;208:49-59.

  11. Nomura LE, Walker JM, Maecker HT. Optimization of whole blood antigen-specific cytokine assays for CD4+ T cells. Cytometry. In press. 2000;40:60-68.

  12. Pitcher CJ, Quittner C, Peterson DM, et al. HIV-1–specific CD4+ T cells are detectable in most individuals with active HIV-1 infection, but decline with prolonged viral suppression. Nat Med. 1999;5:518-525.

  13. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J Immunol Methods. 1998;212:89-98.

  14. Waldrop SL, Davis KA, Maino VC, Picker LJ. Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis. J Immunol. 1998;161:5284-5295.

  15. Waldrop SL, Pitcher CJ, Peterson DM, Maino VC, Picker LJ. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest. 1997;99:1739-1750.

品牌介绍
BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。BD公司具有强大的研发能力和世界上最棘手的多种疾病进行斗争的能力。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务遍及全球。公司的业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。公司的服务对象包括医疗机构,生命科学研究所,临床实验室,工业单位和普通大众。BD has state-of-the-art facilities around the globe that provide an environment that enables our highly talented workforce to be the best at their professions. We hire associates who have a passion and commitment to advancing the world of health. We are always seeking great people to join our company on its journey to greatness.BDRat Granulocytes Pure RP-1  500ug550002BDMs Ig Kpa Lgt Chain FITC 187.1  500ug550007BDICC Fixation Buffer 100mL550011BDMs CD210 NALE 1B1.3a 500ug550019BDMs CD51 Pure RMV-7 100ug550026BDMs CD5 APC 53-7.3 100ug550037BDHu CD62E PE-Cy5 68-5H11 100Tst550041BDHu CD95 Pure EOS9.1  100ug550052BDHam IgG2 Kpa ItCl FITC B81-3 250ug550057BDMs Crry/p65 Pure 1F2 100ug550059BDMs IL-4 Recom Protein 10ug550068BDMs IL-2 Recom Protein 20ug550070BDHu IL-6 Recom Protein 10ug550072BDHu MIP-1 Bta PE D21-1351 100ug550082BDMs IgG1 PE A85-1 100ug550084BDHam IgG2 Kpa ItCl PE B81-3 100ug550086BDHu CD142 Pure HTF-1 100ug550254BDHu CD1d PE CD1d42 100Tst550256BDHu CD73 PE AD2 100Tst550258BDHu CD103 FITC Ber-ACT8 100Tst550260BDMs CD11c APC HL3 100ug550268BDMs CD72b/c Aloatg PE JY/93 100ug550270BDRat CD32 Pure D34-485 500ug550272BDRat CD32 NALE D34-485 500ug550274BDMs CD3e IHC Pure 145-2C11 1mL550277BDMs CD4 IHC Pure H129.19 1mL550280BDMs CD8a IHC Pure 53-6.7 1mL
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