Description
The X40 hybridoma was derived from the fusion of mouse Sp2/0-Ag 14 myeloma cells with spleen cells from BALB/c mice immunized with keyhole limpet hemocyanin (KLH). The hybridoma secretes monoclonal antibody that binds specifically to KLH, an antigen not expressed on human cells or human cell lines.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm.BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter).Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor® 700 detectors.However, the spillover can be corrected through compensation as with any other dye combination.
Format
- FormatBV650
- Excitation SourceViolet 405 nm
- Excitation Max407nm
- Emission Max650nm
BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Other Formats→
- APC-H7
- APC-R700
- Alexa Fluor® 488
- BB515
- BB700
- BUV395
- BUV615
- BV421
- BV480
- BV510
- BV605
- BV711
- BV750
- BV786
- PE-CF594
- PerCP-Cy™5.5
- R718
- V450
- V500
Suggested Companion Products
Stain Buffer (FBS) RUO 500mLCat No: 554656
| Resources & Tools | ||||||
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| SpectrumViewer | Download MSDS | Download TDS | ||||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with BD Horizon™ BV650 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV650 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Brilliant Violet™ 650 is a trademark of Sirigen.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
