Description
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as determined by flow cytometry, can reveal useful information about the cell cycle and the proteins involved in the regulation of the cell cycle. Cells in G2 and M phases of the cell cycle have double the DNA content of those in G0 and G1 phases. Cells in S phase have a DNA content lying between these extremes. PI is detected in the orange range of the spectrum using a 562-588 nm band pass filter.This reagent may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for examining the expression of proteins during the cell cycle.
Suggested Companion Products
Stain Buffer (FBS) RUO 500mLCat No: 554656
Fixation Buffer RUO 100mLCat No: 554655
Perm Buffer II RUO 125mLCat No: 558052
Perm Buffer III RUO 125mLCat No: 558050
Stain Buffer (BSA) RUO 500mLCat No: 554657
| Resources & Tools | ||||||
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| SpectrumViewer | Download MSDS | Download TDS | ||||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.Do not dilute or boil.
Product Notices
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Avoid contact with skin and eyes.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Flow cytometry: After fixing and permeabilizing your cell sample, use 0.5 mL /test (1 x 10e6 cells) and incubate for 15 minutes at room temperature before analysis.Please refer to http://www.bdbiosciences.com/documents/BD_FlowCytometry_DNA_Staining_Protocol.pdf for more protocol information.
