Description
Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cγ. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos.
This antibody is routinely tested by western blot analysis.Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Format
- FormatPurified
Other Formats→
- FITC
Suggested Companion Products
A431 Cell Lysate RUO 500µgCat No: 611447
FITC Goat Anti-Mouse Ig PolyclonalRUO 0.5mgCat No: 554001
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
