Description
The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling.The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase.It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway.However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity.It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ.
Format
- FormatPurified
Suggested Companion Products
HeLa Cell Lysate RUO 500µgCat No: 611449
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
FITC Goat Anti-Mouse Ig PolyclonalRUO 0.5mgCat No: 554001
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Preparation and Storage
Store undiluted at -20°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Western blot:Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
