Description
The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol.The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C.Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains.These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation.It exists as two isoforms: 1) PLCγ1, which is ubiquitously expressed, and 2) PLCγ2, found primarily in the lymphoid system.PLCγ is essential for growth factor-induced cell motility and mitogenesis.PLCγ1 null mice exhibit retarded embryonic growth and lethality in midgestation.Overexpression of PLCγ is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation.Thus regulation of PLCγ activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCγ1, regardless of phosphorylation status.It does not cross-react with PLCγ2.
Format
- FormatPurified
Other Formats→
- Alexa Fluor® 488
- Alexa Fluor® 647
- PE
Suggested Companion Products
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
A431 Cell Lysate RUO 500µgCat No: 611447
FITC Goat Anti-Mouse Ig PolyclonalRUO 0.5mgCat No: 554001
PE Mouse anti-PLCγ1 10/PLCgammaRUO 50TestsCat No: 558575
Alexa Fluor® 488 Mouse anti-PLCγ1 10/PLCgammaRUO 50TestsCat No: 558566
Alexa Fluor® 647 Mouse anti-PLCγ1 10/PLCgammaRUO 50TestsCat No: 558565
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| SpectrumViewer | Download TDS | Regulatory Document Website | ||||
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Western blot:Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
