Description
The 240-kDa dimeric fibronectin protein exists in two forms: a soluble protomer in body fluids and an insoluble multimer in the extracellular matrix. The latter is the primary functional form and creates a substrate for cell migration, a role which makes fibronectin vital to embryogenesis and wound response. Fibronectin mediates cytoskeletal organization, cell attachment, and cellular signaling through interactions with cellular receptors. Although various isoforms of fibronectin are derived by alternative splicing, they share a common N-terminus which is a critical region for cell surface binding in an initial step of multimer assembly. Further polymerization steps are regulated by fibronectin/integrin interactions and result in generation of the complex fibrils that constitute the fibronectin matrix.
Format
- FormatPurified
Other Formats→
- Alexa Fluor® 488
- Alexa Fluor® 647
Suggested Companion Products
A431 Cell Lysate RUO 500µgCat No: 611447
FITC Goat Anti-Mouse Ig PolyclonalRUO 0.5mgCat No: 554001
Fixation Buffer RUO 100mLCat No: 554655
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
Perm Buffer III RUO 125mLCat No: 558050
Perm/Wash Buffer I RUO 125mLCat No: 557885
Purified Mouse IgG1, κ Isotype Control RUO (GMP) 100Cat No: 349040
| Resources & Tools | ||||||
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| SpectrumViewer | Download TDS | Regulatory Document Website | ||||
Preparation and Storage
Store undiluted at -20°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Species testing during development may have been performed with a different format of the same clone.Selected applications have been tested for cross-reactivity.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Triton is a trademark of the Dow Chemical Company.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
