Description
The JES3-19F1 antibody reacts with human interleukin-10 (IL-10). The immunogen used to generate the JES3-19F1 hybridoma was recombinant human IL-10 expressed in COS cells. This is a neutralizing antibody. This antibody also reacts with viral IL-10.
Format
- FormatPurified
Other Formats→
- APC
- BB700
- NA/LE
- PE
- PE-CF594
Suggested Companion Products
BD Cytofix/Cytoperm Plus Kit (with BD GolgiStop) RUO 250TestsCat No: 554715
Purified NA/LE Rat Anti-Human IL-10 JES3-19F1RUO 0.5mgCat No: 554703
Purified Rat Anti-Human IL-10 JES3-19F1RUO 0.5mgCat No: 554705
Recombinant Human IL-10 RUO 5µgCat No: 554611
Protein Transport Inhibitor (Containing Monensin) RUO 0.7mLCat No: 554724
PE Rat Anti-Human IL-10 JES3-19F1RUO 0.1mgCat No: 554706
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
1. Blocking Control for Intracellular Staining: The purified JES3-19F1 antibody can be used as a blocking control to demonstrate specificity of IL-10 staining by PE-JES3-19F1 (Cat. No. 554706). To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled JES3-19F1 antibody (Cat. No. 554704) for 20 minutes at 4°C, prior to staining with PE-JES3-19F1 antibody ( e.g., 0.1 -0.5 µg mAb/1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on intracellular staining in the Immune Function Handbook.
Please note that this application is not routinely tested at BD Biosciences.
2. ELISA Capture: The purified JES3-19F1 antibody is useful as a capture antibody for a sandwich ELISA for specifically measuring human IL-10 protein levels. Purified JES3-19F1 antibody can be paired with the biotinylated JES3-12G8 antibody (Cat. No. 554499) as the detection antibody and with recombinant human IL-10 protein (Cat. No. 554611) as the standard. For testing IL-10 in serum or plasma, our OptEIA™ set (Cat. No. 555134) is recommended.
3. Neutralization: The NA/LE™ format of clone JES3-19F1 (Cat. No. 554703) is useful for neutralization of human IL-10 bioactivity. A suitable NA/LE rat IgG2a isotype control to match the NA/LE JES3-19F1 antibody is the R35-95 antibody, (Cat. No. 554687).
