Description
The monoclonal antibody 4D5/A7/C5 reacts with human CXC chemokine, interferon gamma inducible protein (IP-10). IP-10 is inducible in monocytes, keratinocytes, and endothelial cells by IFN-γ.
This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Format
- FormatPurified
Suggested Companion Products
Biotin Mouse Anti-Human IP-10 6D4/D6/G2RUO 0.5mgCat No: 555048
Recombinant Human IP-10 RUO 10µgCat No: 551130
Human IP-10 ELISA Set RUO 20Plate(s)Cat No: 550926
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
ELISA Capture: The purified monoclonal antibody 4D5/A7/C5 is useful as a capture antibody in a sandwich ELISA for measuring human IP-10 protein levels. Purified monoclonal antibody 4D5/A7/C5 can be paired with the biotinylated monoclonal antibody 6D4/D6/G2 (Cat. No. 555048) with recombinant human IP-10 (Cat. No. 551130) as the standard. Purified monoclonal antibody 4D5/A7/C5 should be titrated to determine its optimal concentration for ELISA capture (0.5-2.0 µg/ml). To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~2500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, w ww.bdbiosciences.com.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested (human IL-1α, IL-1αβ, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, lymphotactin, MIG, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TGF, TNF, LT-β and mouse Crg-2).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring IP-10 in serum or plasma the BD OptEIA™ Human IP-10 ELISA Set (Cat. No. 550926 ) is specially formulated and recommended.
