Description
The MP1-22E9 monoclonal antibody specifically binds to mouse Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF). The immunogen used to generate the MP1-22E9 hybridoma was yeast-expressed recombinant mouse GM-CSF. This is a neutralizing antibody.
Format
- FormatPurified
Other Formats→
- BV421
- NA/LE
- PE
Suggested Companion Products
Recombinant Mouse GM-CSF RUO 10µgCat No: 554586
Biotin Rat Anti-Mouse GM-CSF MP1-31G6RUO 0.5mgCat No: 554407
Mouse GM-CSF ELISA Set RUO 20Plate(s)Cat No: 555167
Purified NA/LE Rat Anti-Mouse GM-CSF MP1-22E9RUO 0.5mgCat No: 554403
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
ELISA Capture: The purified MP1-22E9 antibody (Cat. No. 554404) is useful as a capture antibody for a sandwich ELISA for measuring mouse GM-CSF protein levels. Purified MP1-22E9 antibody can be paired with the biotinylated MP1-31G6 (Cat. No. 554407) antibody as the detecting antibody, with recombinant mouse GM-CSF (Cat. No. 554586) as the standard. Purified MP1-22E9 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse GM-CSF ranging from ~2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1ß, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring GM-CSF in serum or plasma our mouse GM-CSF BD OptEIA™ set (Cat. No. 555167) is specially formulated and recommended.
Western Blot:The MP1-22E9 antibody (Cat. 554404) has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Neutralization: The NA/LE™ MP1-22E9 antibody (Cat. No. 554403) is useful for neutralization of mouse GM-CSF bioactivity.
Immunofluorescent Staining and Flow Cytometric Analysis: The MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify and enumerate GM-CSF producing cells within mixed cell populations. The PE-conjugated MP1-22E9 antibody (Cat. No. 554406) is especially suitable for these experiments.
