Description
Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.
Streptavidin was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm.BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter).Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor® 700 detectors.However, the spillover can be corrected through compensation as with any other dye combination.
Format
- FormatBV650
- Excitation SourceViolet 405 nm
- Excitation Max407nm
- Emission Max650nm
BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Other Formats→
- AKP
- APC
- APC-Cy™7
- APC-R700
- BB515
- BUV395
- BUV496
- BUV563
- BUV615
- BUV661
- BUV737
- BUV805
- BV421
- BV480
- BV510
- BV605
- BV711
- BV786
- FITC
- HRP
- PE
- PE-CF594
- PE-Cy™5
- PE-Cy™7
- PerCP
- PerCP-Cy™5.5
- V450
- V500
Suggested Companion Products
Stain Buffer (FBS) RUO 500mLCat No: 554656
Lysing Buffer RUO 100mLCat No: 555899
Biotin Rat Anti-Mouse CD45R/B220 RA3-6B2RUO 0.1mgCat No: 553085
Stain Buffer (BSA) RUO 500mLCat No: 554657
Biotin Rat Anti-Mouse CD45R/B220 RA3-6B2RUO 0.5mgCat No: 553086
Brilliant Stain Buffer RUO 1000TestsCat No: 566349
Brilliant Stain Buffer RUO 100TestsCat No: 563794
Brilliant Stain Buffer Plus RUO 1000TestsCat No: 566385
| Resources & Tools | ||||||
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| Spectrum Viewer | Panel Designer | SpectrumViewer | Download TDS | Regulatory Document Website | ||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon™ BV650 Streptavidin is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.
