Description
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Format
- FormatFITC
- Excitation SourceBlue 488 nm
- Excitation Max494nm
- Emission Max520nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats→
- AKP
- APC
- APC-Cy™7
- APC-R700
- BB515
- BUV395
- BUV496
- BUV563
- BUV615
- BUV661
- BUV737
- BUV805
- BV421
- BV510
- BV605
- BV650
- BV711
- BV786
- HRP
- PE
- PE-CF594
- PE-Cy™5
- PE-Cy™7
- PerCP
- PerCP-Cy™5.5
- V450
- V500
Suggested Companion Products
Stain Buffer (FBS) RUO 500mLCat No: 554656
Stain Buffer (BSA) RUO 500mLCat No: 554657
Resources & Tools | ||||||
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Spectrum Viewer | Panel Designer | SpectrumViewer | Download MSDS | Download TDS |
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.