Description
Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.
Streptavidin was conjugated to BD Horizon BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.
Format
- FormatBUV805
- Excitation SourceUltraviolet 355 nm
- Excitation Max348nm
- Emission Max805nm
BUV805 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an Em Max at 805 nm. BUV805 has been exclusively developed by BD Biosciences for instruments equipped with a 355-nm UV laser.
Other Formats→
- AKP
- APC
- APC-Cy™7
- APC-R700
- BB515
- BUV395
- BUV496
- BUV563
- BUV615
- BUV661
- BUV737
- BV421
- BV510
- BV605
- BV650
- BV711
- BV786
- FITC
- HRP
- PE
- PE-CF594
- PE-Cy™5
- PE-Cy™7
- PerCP
- PerCP-Cy™5.5
- V450
- V500
Suggested Companion Products
Biotin Rat Anti-Mouse CD45R/B220 RA3-6B2RUO 0.1mgCat No: 553085
Biotin Rat Anti-Mouse CD45R/B220 RA3-6B2RUO 0.5mgCat No: 553086
Stain Buffer (FBS) RUO 500mLCat No: 554656
Stain Buffer (BSA) RUO 500mLCat No: 554657
Lysing Buffer RUO 100mLCat No: 555899
Brilliant Stain Buffer RUO 1000TestsCat No: 566349
Brilliant Stain Buffer RUO 100TestsCat No: 563794
Brilliant Stain Buffer Plus RUO 1000TestsCat No: 566385
| Resources & Tools | ||||||
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| Spectrum Viewer | Panel Designer | SpectrumViewer | Download TDS | Regulatory Document Website | ||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.Streptavidin was conjugated with BD Horizon BUV805 under optimum conditions, and unconjugated streptavidin and free BD Horizon BUV805 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- BD Horizon Brilliant Ultraviolet 805 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
BD Horizon™ BUV805 Streptavidin is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.
