Description
IgM is an important component in the first line of defense against foreign pathogens, but may also play a role in autoimmune diseases.IgM monomers consist of two light and two heavy chains.Unlike the heavy chain of an IgG antibody which contains 3 constant Ig domains, the µ heavy chain of IgM contains 4 constant Ig domains.Five IgM monomers complex with a small polypeptide (J-chain) to form pentameric IgM that can be found in human plasma.In an immune response, the binding of IgM to a cell surface antigen enables C1q to activate interactions with downstream components in the classical complement pathway.Mature B lymphocytes express IgM.The G20-127 monoclonal antibody binds to the heavy chain of human IgM. The G20-127 antibody is not thought to react with other immunoglobulin heavy chain isotypes.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
Format
- FormatBV510
- Excitation SourceViolet 405 nm
- Excitation Max405nm
- Emission Max510nm
BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
Other Formats→
- APC
- BB515
- BUV395
- BV421
- BV480
- BV605
- BV650
- BV711
- BV786
- Biotin
- FITC
- PE
- PE-CF594
- PE-Cy™5
- PerCP-Cy™5.5
- Purified
- V450
Suggested Companion Products
APC Mouse Anti-Human CD19 HIB19RUO 100TestsCat No: 555415
APC Mouse Anti-Human CD19 HIB19RUO 25TestsCat No: 561742
Stain Buffer (FBS) RUO 500mLCat No: 554656
BV510 Mouse IgG1, k Isotype Control RUO 50µgCat No: 562946
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test.We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Brilliant Violet™ 510 is a trademark of Sirigen.
