Description
The R9-91 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R9-91 mAb.
Format
- FormatPurified
Suggested Companion Products
Biotin Rat Anti-Mouse IgG2b R12-3RUO 0.5mgCat No: 553393
Purified Mouse IgG2b, κ Isotype Control RUO 0.5mgCat No: 557351
FITC Rat Anti-Mouse IgG2b R12-3RUO 0.5mgCat No: 553395
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For the sandwich mouse IgG2b ELISA, purified mAb R9-91 is optimal for capture with biotin anti-mouse IgG2b R12-3 mAb (Cat. No. 553393) for detection and Cat. No. 557351 as a standard. Purified R9-91 mAb may be used as a primary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC-conjugated mAb R12-3 (Cat. No. 553395).