Description
The R11-89 antibody recognizes mouse IgG2a, with strong reactivity to the Igh-1[a] allotype and weaker reactivity to Igh-1[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
Format
- FormatPurified
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For the sandwich IgG2a ELISA, R11-89 mAb is optimal for capture with biotin-, AKP-, or HRP-conjugated anti-mouse IgG2a R19-15 mAb (Cat. No. 553388, 553389, or 553391, respectively) for detection. This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C haplotypes, in order of sensitivity from highest to lowest: e, a, j, and b.
