Description
The R12-3 antibody recognizes an epitope inthe CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.
Format
- FormatPurified
Other Formats→
- BB700
- BUV395
- BUV496
- BUV615
- BUV661
- BV421
- BV480
- BV510
- BV605
- BV650
- BV711
- BV750
- BV786
- Biotin
- FITC
Suggested Companion Products
FITC Rat Anti-Mouse IgG2b R12-3RUO 0.5mgCat No: 553395
Biotin Rat Anti-Mouse IgG2b R12-3RUO 0.5mgCat No: 553393
Purified Rat Anti-Mouse IgG2b R9-91RUO 0.5mgCat No: 553396
Resources & Tools | ||||||
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SpectrumViewer | Download TDS | Regulatory Document Website |
Preparation and Storage
Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Forthe sandwich mouse, Biotin Rat Anti-Mouse IgG2b(Cat. No. 553393) is optimal for detection with Purified Rat Anti-Mouse IgG2b (Cat. No. 553396) for capture. Purified Rat Anti-Mouse IgG2b may be used as a primary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC Rat Anti-Mouse IgG2b (Cat. No. 553395).