Description
The G20-193 monoclonal antibody specifically binds to human immunoglobulin light chain, kappa (κ). It does not react with human immunoglobulin λ light chains or human immunoglobulin heavy chains.
Format
- FormatAPC
- Excitation SourceRed 633 nm
- Excitation Max650nm
- Emission Max660nm
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Other Formats→
- APC-H7
- Alexa Fluor® 700
- BB515
- BB700
- BUV395
- BUV496
- BUV615
- BUV661
- BV421
- BV480
- BV510
- BV605
- BV650
- BV711
- BV750
- BV786
- Biotin
- FITC
- PE
- PE-CF594
- PE-Cy™7
- PerCP-Cy™5.5
- Purified
- V450
Suggested Companion Products
APC Mouse IgG1 κ Isotype Control RUO 0.1mgCat No: 554681
Stain Buffer (FBS) RUO 500mLCat No: 554656
Alexa Fluor® 488 Mouse Anti-Human CD19 HIB19RUO 100TestsCat No: 557697
| Resources & Tools | ||||||
|---|---|---|---|---|---|---|
| Spectrum Viewer | Panel Designer | SpectrumViewer | Download MSDS | Download TDS | ||
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test.We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
