BD Biosciences/Mouse T Lymphocyte Subset Antibody Cocktail, with Isotype Control; PE-Cy™7 CD3e, PE CD4, and APC CD8a/100Tests/558431-免疫在线蚂蚁淘旗下平台

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商品详细BD Biosciences/Mouse T Lymphocyte Subset Antibody Cocktail, with Isotype Control; PE-Cy™7 CD3e, PE CD4, and APC CD8a/100Tests/558431

BD Biosciences/Mouse T Lymphocyte Subset Antibody Cocktail, with Isotype Control; PE-Cy™7 CD3e, PE CD4, and APC CD8a/100Tests/558431

商品编号： 558431

品牌： bdbiosciences

市场价： ¥148533.00

美元价： 84876.00

产地：美国(厂家直采)

公司：

产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Product DetailsRecommended AssayReferences

Description

The Mouse T Lymphocyte Subset Antibody Cocktail is a three-color reagent designed to identify major subsets of T lymphocytes by direct immunofluorescent staining with flow cytometric analysis. This cocktail consists of the following antibody mixture: PE-Cy7 hamster anti-mouse CD3e (clone 145-2C11), PE rat anti-mouse CD4 (clone RM4-5), and APC rat anti-mouse CD8a (clone 53-6.7).The 145-2C11 antibody reacts with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells of all mouse strains tested. The RM4-5 antibody recognizes CD4 (L3T4), a differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T-helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells in all mouse strains tested. CD4 has also been detected on pluripotent hematopoietic stem cells, bone-marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. The 53-6.7 antibody reacts with the 38-kDa α and 34-kDa α" chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) expressed on most thymocytes, subpopulations of mature T lymphocytes (including MHC class I-restricted T suppressor/cytotoxic cells and subsets of γδ TCR-bearing cells and intestinal intraepithelial lymphocytes) of all mouse strains tested. CD8a is also expressed on a subset of dendritic cells. The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance. The Mouse T Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulin.

The use of three different fluorochromes for the labeling of the three different antibodies permits the recognition of each of the three antigens on each cell in a sample. The levels of expression of the three antigens distinguish the major subpopulations of developing and peripheral T lymphocytes. Additional fluorochrome-labeled reagents may be combined with the Mouse T Lymphocyte Subset Antibody Cocktail, and the Mouse T Lymphocyte Subset Isotype Control, to further characterize T-cell subpopulations.

The Mouse T Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched

negative-control immunoglobulin consisting of the following: PE-Cy7 Armenian hamster IgG1, κ (clone A19-3),and PE and APC Rat IgG2a, κ (clone R35-95).

Components

Part No.	Description	Size
51-9000790	Mouse T Lymphocyte Subset Antibody Cocktail; PE-Cy™7 CD3e, PE CD4, and APC CD8a Aqueous buffered solution containing BSA and ≤0.09% sodium azide.;Volume Per Test:20 µl	100Tests (1 ea)
51-9000792	Mouse T Lymphocyte Subset Isotype Control; PE-Cy™7 Hamster IgG1, κ, PE & APC Rat IgG2a, κ Aqueous buffered solution containing BSA and ≤0.09% sodium azide.;Volume Per Test:20 µl	100Tests (1 ea)

Resources & Tools
SpectrumViewer	Download TDS	Regulatory Document Website

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.

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Godfrey DI, Kennedy J, Mombaerts P, Tonegawa S, Zlotnik A. Onset of TCR-β gene rearrangement and role of TCR-β expression during CD3-CD4-CD8- thymocyte differentiation. J Immunol. 1994; 152(10):4783-4792.View reference
Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979; 47:63-90.View reference
Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980; 152(2):280-295.View reference
Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981; 153(6):1503-1516.View reference
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Martin P, del Hoyo GM, Anjuere F, et al. Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors. Blood. 2000; 96(7):2511-2519.View reference
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品牌介绍

BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗，提高传染性疾病诊断的质量和速度，推进新型药物和疫苗的研究与发现。BD公司具有强大的研发能力和世界上最棘手的多种疾病进行斗争的能力。公司于1897年在纽约成立，总部位于美国新泽西州的富兰克林湖，业务遍及全球。公司的业务可分为BD医疗、BD诊断、BD生物科学三大类，生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。公司的服务对象包括医疗机构，生命科学研究所，临床实验室，工业单位和普通大众。BD has state-of-the-art facilities around the globe that provide an environment that enables our highly talented workforce to be the best at their professions. We hire associates who have a passion and commitment to advancing the world of health. We are always seeking great people to join our company on its journey to greatness.BDRat Granulocytes Pure RP-1 500ug550002BDMs Ig Kpa Lgt Chain FITC 187.1 500ug550007BDICC Fixation Buffer 100mL550011BDMs CD210 NALE 1B1.3a 500ug550019BDMs CD51 Pure RMV-7 100ug550026BDMs CD5 APC 53-7.3 100ug550037BDHu CD62E PE-Cy5 68-5H11 100Tst550041BDHu CD95 Pure EOS9.1 100ug550052BDHam IgG2 Kpa ItCl FITC B81-3 250ug550057BDMs Crry/p65 Pure 1F2 100ug550059BDMs IL-4 Recom Protein 10ug550068BDMs IL-2 Recom Protein 20ug550070BDHu IL-6 Recom Protein 10ug550072BDHu MIP-1 Bta PE D21-1351 100ug550082BDMs IgG1 PE A85-1 100ug550084BDHam IgG2 Kpa ItCl PE B81-3 100ug550086BDHu CD142 Pure HTF-1 100ug550254BDHu CD1d PE CD1d42 100Tst550256BDHu CD73 PE AD2 100Tst550258BDHu CD103 FITC Ber-ACT8 100Tst550260BDMs CD11c APC HL3 100ug550268BDMs CD72b/c Aloatg PE JY/93 100ug550270BDRat CD32 Pure D34-485 500ug550272BDRat CD32 NALE D34-485 500ug550274BDMs CD3e IHC Pure 145-2C11 1mL550277BDMs CD4 IHC Pure H129.19 1mL550280BDMs CD8a IHC Pure 53-6.7 1mL

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