Description
The Golgi apparatus is a collection of flattened membranes, or cisternae, and vesicles. Protein maturation and post translational modification occur after their biosynthesis at the ER and transport through the Golgi apparatus. This involves the transport of protein containing vesicles through a vectorial process of vesicle budding and fusion from the cis-compartment (CGN) to the medial-compartment (MGN) and the trans-compartment (TGN) of the Golgi apparatus. Various proteins resident to specific Golgi compartments are necessary for proper function. TGN38 is a type I integral membrane protein primarily localized to the TGN. It is involved in the sorting of nascent proteins into carrier vesicles for transport to appropriate destinations. It is thought to heterodimerize with TGN41 and participate in exocytic budding. GS28 (Golgi SNARE 28 kDa) is associates with the cis-Golgi and participates in trafficking between the ER and the Golgi and between Golgi compartments. GS28 and syntaxin 5 exist as a Golgi complex, which is important for the function of both proteins in ER-Golgi transport. GM130 (Golgi matrix protein of 130 kDa) is present in the Golgi matrix and peripherally associated with the cis-compartment, where it colocalizes with syntaxin5. GM130 is homologous to the Golgi auto-antigen golgin-95 and interacts through its N-terminal domain with p115 and with the Golgi membranes through its C-terminal portion. GM130 functions as a structural element of the Golgi apparatus and also provides attachment sites for membranes and other Golgi proteins. Thus, the Golgi system is essential for the modification, sorting, and packaging of proteins and other macromolecules for delivery within or secretion from the cell.
Investigators should note that the antibody to Syntaxin-6 has not been evaluated for any potential crossreactivity to other syntaxin isoforms.
Suggested Companion Products
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
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Preparation and Storage
Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Western blot:Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
