Description
All cells, both prokaryotic and eukaryotic, contain phospholipid bilayer membranes.However, eukaryotic cells also contain an extensive and intricate internal array of membranes.These membranes form a subcellular set of structures called the organelles, which enclose specific regions and separate them from the remainder of the cytoplasm.The most prominent organelle is the nucleus, which partitions the genetic.Located in structural continuity with the nuclear outer membrane is the rough endoplasmic reticulum (ER), the site of protein synthesis.Smooth ER is found throughout the cytoplasm and is the site of fatty acid and phospholipid metabolism.Newly synthesized protein is transported, via vesicles, from the rough ER to the Golgi apparatus.The Golgi is a system of stacked, flattened sacks that function in modifying, sorting, and packaging proteins and other macromolecules for delivery within the cell or for secretion from the cell.Other prominent organelles are the mitochondria, which are thought to have evolved from bacteria that were engulfed by cells with which they initially lived in symbiosis.Mitochondria are the site of oxidative phosphorylation reactions that result in the production of cellular ATP.The endocytic pathway involves other organelles, such as the endosomes and lysosomes.Newly ingested material is contained within the endosome which, in turn, passes the material to lysosomes for degradation by the digestive enzymes that are active in the acidic lysosomal lumen.Each organelle and its associated proteins perform distinct functions that are essential for cellular growth and metabolism.The following antibodies in this sampler kit may be useful for examining the following organelles:
Annexin II= plasmalemaBiP/GRP78= endoplasmic reticulumâ-Catenin= zonula adherensCaveolin-1= caveolae
Connexin-43= gap junctionsEEA1= endosomesGM130= GolgiLamp-1= lysosomes
MAP2B= microtubulesBcl-2= mitochondriaNucleoporin p62= nucleusPaxillin= focal contacts
CD49b (Integrin α2)= plasma membraneZO-1= zonula occludens
Suggested Companion Products
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
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Preparation and Storage
Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Western blot:Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp
