Description
p53 is a nuclear phosphoprotein that plays a key role in cell growth regulation, particularly inhibition of cell proliferation. BD Biosciences Pharmingen offers a panel of p53 specific antibodies. The recombinant p53 protein is useful as a western blot control for antibodies that recognize human p53 and work in western blot. These antibodies include the following p53 clones: G59-12, PAb 122, PAb 240, PAb 1801, DO-1, DO-7. Recombinant human p53 was expressed in the Baculovirus Expression Vector System (BEVS) and purified from Sf9 insect cell lysate using an anti-p53 antibody, clone DO-7 (Cat. No. 554294).
The purity and the integrity of the protein was verified by SDS-PAGE and Western blot analysis.
Suggested Companion Products
Purified Mouse Anti-Human p53 G59-12RUO 0.1mgCat No: 554157
Purified Mouse Anti-p53 PAb 122RUO 0.1mgCat No: 554147
Purified Mouse Anti-p53 PAb 240RUO 0.1mgCat No: 554166
Purified Mouse Anti-p53 PAb 240RUO 0.25mgCat No: 554167
Purified Mouse Anti-Human p53 PAb 1801RUO 0.1mgCat No: 554169
Purified Mouse Anti-Human p53 DO-1RUO 0.1mgCat No: 554293
Purified Mouse Anti-Human p53 DO-7RUO 0.1mgCat No: 554294
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Preparation and Storage
This preparation contains no preservatives, thus it should be handled under aseptic conditions.Avoid multiple freeze-thaws of product.The protein solution was sterile filtered and frozen at -20°C. Store the protein at -20°C; it may also be stored at -80°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
1 µg of protein can easily be visualized on a Coomassie Blue stained SDS/PAGE as a band of 53 kDa. For Western blot analysis, 50 ng or less is sufficient for the chemiluminescence detection method. The p53 recombinant protein has not been evaluated for suitability in other assays outside of Western blot analysis.
