Description
Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel.
Suggested Companion Products
Mouse Macrophage Cell Lysate RUO 500µgCat No: 611479
Purified Mouse Anti-Cox-2 33/Cox-2RUO 50µgCat No: 610203
Purified Mouse Anti-iNOS/NOS Type II 6/iNOS/NOS Type IIRUO 50µgCat No: 610328
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Preparation and Storage
Do not dilute or boil.Store undiluted at -20°C.Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10 ng/ml IFNγ and 1 µg/ml LPS for 12 hours.Western blots of mouse macrophages stimulated with IFNγ and LPS are evaluated for the induction of iNOS and Cox-2 using monoclonal antibodies Cat. No. 610328 and 610203 respectively. Unstimulated mouse macrophages (Cat. No. 611479) show no expression of iNOS or Cox-2.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Warning: BD Transduction Laboratories™ Mouse Macrophage + IFNγ/LPS Lysate contains 12.171% 1,2,3-Propanetriol and 2.068% sodium dodecyl sulfate (w/w).
Hazard Statements:
Causes serious eye irritation.
Precautionary Statements:
Wear protective gloves/protective clothing/eye protection/face protection.
Wash thoroughly after handling.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.
