Description
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
Format
- FormatAlexa Fluor® 647
- Excitation SourceRed 633 nm
- Excitation Max650nm
- Emission Max668nm
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
Other Formats→
- APC
- APC-Cy™7
- APC-H7
- Alexa Fluor® 488
- Alexa Fluor® 700
- Biotin
- FITC
- PE
- PE-Cy™5
- PE-Cy™7
- Pacific Blue™
- PerCP
- PerCP-Cy™5.5
- Purified
- V450
Suggested Companion Products
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO 100TestsCat No: 557714
Stain Buffer (FBS) RUO 500mLCat No: 554656
Perm Buffer III RUO 125mLCat No: 558050
Fixation Buffer RUO 100mLCat No: 554655
| Resources & Tools | ||||||
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| SpectrumViewer | Download MSDS | Download TDS | ||||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- This reagent has been pre-diluted for use at the recommended Volume per Test.We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
For non-intracellular flow applications, investigators are encouraged to try BD Pharmingen™ Alexa Fluor® 647 Mouse IgG1 κ Isotype Control, Clone MOPC-21, Cat. No. 557714.
