Description
The ROCK inhibitor, Y-27632, is a selective inhibitor of the p160-Rho-associated coiled kinase (ROCK) and is a factor that enhances embryonic stem cell survival upon single cell dissociation. In addition to increasing cell recovery after dissociation, Y-27632 has been used for various other applications in stem cell research including cryopreservation, sorting, reprogramming, transplantation, and differentiation.
Suggested Companion Products
Human Pluripotent Stem Cell Sorting And Analysis Kit RUO Cat No: 560461
Accutase™ Cell Detachment Solution RUO 100mLCat No: 561527
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| SpectrumViewer | Download MSDS | Download TDS | ||||
Preparation and Storage
Store at room temperature, desiccated and protected from prolonged exposure to light.Store aliquots of the reconstituted material at -20°C, protected from prolonged exposure to light, and avoid repeated freeze-thaw cycles.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Avoid contact with skin and eyes.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- mTESR™1 is a trademark of StemCell Technologies.
- Accutase is a registered trademark of Innovative Cell Technologies, Inc.
For a working solution, dilute to 10 mM in sterile deionized water. [The molar mass of Y-27632 is 338.3 g/mol.]
Small aliquots may be stored at -20°C, avoiding repeated freeze-thaw cycles.
Use at 10 µM in culture medium. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
