Description
Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction.However, these interactions are mediated by the non-snRNP factors.The super family of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate SR domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPL1 is specific for SR domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, whereas SRPK2 is highly expressed in brain. SRPKs affect splice-site selection and are thought to affect alternative splicing.
Format
- FormatPurified
Suggested Companion Products
HeLa Cell Lysate RUO 500µgCat No: 611449
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
FITC Goat Anti-Mouse Ig PolyclonalRUO 0.5mgCat No: 554001
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
