Description
3-Amino-9-Ethylcarbazole (AEC) is a substrate for peroxidase and is a widely used chromogen for immunohistochemical staining and immunoblotting. In the presence of peroxidase enzyme, AEC produces a red end product that is soluble in alcohol and therefore must be used with an aqueous counterstain and mounting media.
Components
| Part No. | Description | Size |
|---|---|---|
| 51-7604KC | AEC Chromogen (component of AEC substrate Kit) | 1.5mL (1 ea) |
| 51-7603KC | AEC buffer (component of AEC substrate Kit) | 50mL (1 ea) |
| Resources & Tools | ||||||
|---|---|---|---|---|---|---|
| SpectrumViewer | Download TDS | Download MSDS | ||||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.
Product Notices
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Immunohistochemistry: Just before staining add one drop of AEC chromogen for every 1 mL of AEC buffer. Apply the AEC substrate reagent to the sections after incubation with horseradish peroxidase and washing is complete. Incubate sections with AEC reagent for 5-20 minutes or until the desired color intensity is obtained. Use the AEC substrate reagent within 20 minutes of mixing.
Warning:AEC buffer (component 51-7603KC) contains 7.99% methanol (w/w).
Hazard statements
Combustible liquid.
Harmful if swallowed.
May be harmful in contact with skin.
May cause damage to organs.
Precautionary statements
Wear protective gloves / eye protection.
Wear protective clothing.
Avoid breathing mist/vapours/spray.
IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
IF ON SKIN: Wash with plenty of water.
Warning:AEC Chromogen (component 51-7604KC) contains 97.8% N,N-dimethylformamide (w/w).
Hazard statements
Flammable liquid and vapour.
Harmful if inhaled.
Causes serious eye irritation.
May damage fertility or the unborn child.
Precautionary statements
Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
Wear protective clothing / eye protection.
Wear protective gloves.
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.
Dispose of contents/container in accordance with local/regional/national/international regulations.
