Description
BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins.BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer.It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies.BD Phosflow™ Perm/Wash Buffer I is provided as a10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer.Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.
Suggested Companion Products
Fix Buffer I RUO 250mLCat No: 557870
Lyse/Fix Buffer 5X RUO 250mLCat No: 558049
Fixation Buffer RUO 100mLCat No: 554655
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Preparation and Storage
Store undiluted at 4°C.Do not freeze.
Product Notices
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Flow cytometry: Dilute BD Phosflow™ Perm/Wash Buffer I1:10 in distilled water prior to use. If desired, the diluted 1X BD™ Phosflow Perm/Wash Buffer I can be passed through a 0.45 µm filter to remove any residual precipitate.The BD Phosflow™ Perm/Wash Buffer I can be used to permeabilize or wash cells and to dilute antibodies for immunofluorescent staining of intracellular proteins.
