Description
The 70-kDa ζ chain-associated protein (ZAP70) is a Syk-family protein tyrosine kinase (PTK) that associates with the ζ subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation.ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus.TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 in the regulatory loop of the PTK domain leading to upregulation of ZAP70 kinase activity.Tyrosine 292 (Y292), in the linker region between the SH2 and PTK domains, is autophosphorylated by the activated PTK domain.By binding with c-Cbl, the phosphorylated Y292 can down-regulate TCR signaling.
The J34-602 antibody recognizes ZAP70 phosphorylated at Y292.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Other Formats→
- Alexa Fluor® 488
- Alexa Fluor® 647
Suggested Companion Products
Fixation Buffer RUO 100mLCat No: 554655
Lyse/Fix Buffer 5X RUO 250mLCat No: 558049
Perm Buffer III RUO 125mLCat No: 558050
Perm Buffer II RUO 125mLCat No: 558052
Perm/Wash Buffer I RUO 125mLCat No: 557885
| Resources & Tools | ||||||
|---|---|---|---|---|---|---|
| Spectrum Viewer | Panel Designer | SpectrumViewer | Download TDS | Regulatory Document Website | ||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test.We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer).Any of the three BD Phosflow™ permeabilization buffers may be used.
