Description
TIAR is an RNA-binding protein related to TIA-1. Both proteins are candidate effectors of apoptotic cell death and consist of three N-terminal RNA-recognition motifs (RRM) and a C-terminal protein-interaction domain (PID). Unlike TIA-1, which is localized in the granules of cytotoxic lymphocytes, TIAR is predominantly found in the nucleus of many different cell types. Two related isoforms (42 kDa and 50 kDa) of TIAR have been identified. It has been demonstrated that TIAR can trigger DNA fragmentation in permeabilized thymocytes, suggesting that it may be an effector of apoptotic cell death. During Fas-mediated apoptosis, TIAR shows a rapid translocation from the nucleus tothe cytoplasm. This redistribution of TIAR appears to be a specific event and possibly a general feature of the apoptotic program.
Format
- FormatPurified
Suggested Companion Products
Mouse Macrophage Cell Lysate RUO 500µgCat No: 611479
HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002
FITC Goat Anti-Mouse Ig PolyclonalRUO 0.5mgCat No: 554001
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Western blot:Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
