Description
The B-K29 monoclonal antibody specifically binds to CD262 which is also known as, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL Receptor 2, TRAIL-R2), Death receptor 5 (DR5), TRICK2, or KILLER. CD262 is a type I transmembrane protein that is encoded by TNFRSF10B (Tumor necrosis factor receptor superfamily, member 10b). CD262 forms a homotrimeric receptor complex that can bind homotrimeric TRAIL (CD253/APO-2 Ligand) and transduce apoptotic signals intracellularly through its cytoplasmic death domain (DD). CD262 is differentially expressed on cells from a wide variety of normal tissues and tumors. CD262 expression is upregulated by Interferon-α (IFN-α).
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm.BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter).Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor® 700 detectors.However, the spillover can be corrected through compensation as with any other dye combination.
Format
- FormatBV650
- Excitation SourceViolet 405 nm
- Excitation Max407nm
- Emission Max650nm
BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Other Formats→
- Alexa Fluor® 647
- BUV395
- BUV615
- BV421
- BV480
- BV510
- BV605
- BV711
- BV786
Suggested Companion Products
Stain Buffer (FBS) RUO 500mLCat No: 554656
Stain Buffer (BSA) RUO 500mLCat No: 554657
Brilliant Stain Buffer RUO 100TestsCat No: 563794
Lysing Buffer RUO 100mLCat No: 555899
Lysing Solution 10X Concentrate CFDA IVD 100Cat No: 349202
Human BD Fc Block™ RUO 50mgCat No: 564219
BV650 Mouse IgG1, k Isotype Control RUO 50µgCat No: 563231
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity.However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
