Description
Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors.In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets.These influences can involve the capacity of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.Under reduced SDS-PAGE conditions, recombinant mouse IFN-γ is a differentially glycosylated protein with a molecular mass of approximately 17 kD.Recombinant mouse IFN-γ is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives.Recombinant mouse IFN-γ
is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.The endotoxin level is ≤ 0.1 ng per µg of mouse IFN-γ, as measured in a chromogenic LAL assay.
Suggested Companion Products
Purified Rat Anti-Mouse IFN-γ R4-6A2RUO 1mgCat No: 551216
Biotin Rat Anti-Mouse IFN-γ XMG1.2RUO 0.5mgCat No: 554410
Mouse IFN-γ ELISA Set RUO 20Cat No: 551866
Mouse IFN-γ ELISA Kit II RUO 2Cat No: 558258
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Upon initial thawing, recombinant mouse IFN-γ (Cat. No. 554587) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use.Alternatively, the product can be diluted in sterile neutral buffer containing not less than 1 - 10 mg/mL carrier protein, such as human or bovine albumin, aliquoted and stored at -80°C.For in vitro biological assay use, carrier protein concentrations of1 - 2 mg/mL are recommended.For use as an ELISA standard, carrier protein concentrations of 5 - 10 mg/mL are recommended.Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.The product should not be diluted to less than 50 µg/mL for long term storage.Carrier proteins should be pre-screened for possible effects in each investigator"s experimental system.Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Stamdard: Recombinant mouse IFN-γ (Cat. No. 554587) can be useful as a quantitative standard for measuring mouse IFN-γ protein levels using sandwich ELISA with the purified R4-6A2 antibody (Cat. No. 551216) as a capture antibody and biotinylated XMG1.2 antibody (Cat. No. 554410) as the detection antibody.To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant mouse IFN-γ standard from 4,000 - 30 pg/mL to be included in each ELISA plate.For measuring mouse IFN-γ in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IFN-γ ELISA Set (Cat. No. 551866) or BD OptEIA™ Mouse IFN-γ Kit
(Cat. No. 558258).
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments.An activity range of 0.3 - 3.3 x 10^8 units/mg, encompassing an
ED50= 30 - 300 pg/mL, has previously been reported using L929 as indicator cells for proliferation, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
