Description
Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of pro-inflammatory T cell mediated immunity. Mouse IL-10 is an 18.8 kD protein containing 160 amino acid residues.Recombinant mouse IL-10 (Cat. No. 550070) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives.Recombinant mouse IL-10 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.The endotoxin level is ≤ 0.1 ng/µg of mouse IL-10, as measured in a chromogenic LAL assay.
Suggested Companion Products
Recombinant Mouse IL-4 RUO 10µgCat No: 550067
Purified Rat Anti-Mouse IL-10 JES5-2A5RUO 1mgCat No: 551215
Biotin Rat Anti-Mouse IL-10 SXC-1RUO 0.5mgCat No: 554423
Biotin Rat Anti-Mouse IL-10 JES5-16E3RUO 0.5mgCat No: 554465
Mouse IL-10 ELISA Set RUO 20Cat No: 555252
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Upon initial thawing, recombinant mouse IL-10 (Cat. No. 550070) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use.Alternatively, the product can be diluted in sterile netural buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C.For use as an ELISA standard, carrier protein concentrations of 5 - 10 mg/mL are recommended.For in vtiro biological assays, carrier protein concentrations of 1 - 2 mg/mL are suggested.Failure to add carrier protein or store at the indicated temperatures may result in a loss of activity.This product should not be diluted to less than 50 µg/mL for long term storage.Carrier proteins should be pre-screened for possible effects in each investigator"s experimental system.Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Mouse IL-10 is useful as a quantitative standard for measuring mouse IL-10 protein levels in an IL-10 specific sandwich ELISA with the purified JES5-2A5 antibody (Cat. No. 551215) as a capture antibody and the biotinylated clone SXC-1 (Cat. No. 554423) or JES-16E3 (Cat. No. 554465) as the detection antibody. To obtain linear standard curves, doubling dilutions of the mouse IL-10 standard from ~2,000 to 15 pg/mL should be included with each ELISA plate.This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems, not for assay of serum or plasma samples. For measuring IL-10 in serum or plasma, the BD OptEIA™ Mouse IL-10 Set (Cat. No. 555252) is specially formulated and recommended.
Bioassay:Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments.An activity range of 1.0 - 6.6 x 10^5 ng/mL, encompassing an
ED50= 0.5 - 100 ng/mL, has previously been reported using D36 indicator cells co-stimulated with recombinant mouse IL-4 (Cat No. 550067) at 50 pg/mL to promote proliferation and were incubated for 24 hours prior to being pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
