Description
Purified Mouse C3a protein is a native protein isolated from activated mouse serum. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation.Mouse C3a protein was isolated from activated mouse serum by affinity chromatography. This product can be reconstituted using 1 mL of Assay Diluent (Cat. No. 555213) or equivalent neutral buffer containing carrier protein, aliquoted into single-use aliquots and stored at -80°C.Avoid multiple freeze-thaws of the reconstituted protein.Each vial contains enough protein for ten 96-well plates.Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.This product should not be diluted prior to long-term storage.
Suggested Companion Products
Purified Rat Anti-Mouse C3a I87-1162RUO 0.1mgCat No: 558250
Biotin Rat Anti-Mouse C3a I87-419RUO 0.1mgCat No: 558251
Assay Diluent RUO 500mLCat No: 555213
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Preparation and Storage
Store the lyophilized standard at 4°C prior to reconstitution.
Product Notices
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- ProClin is a trademark of Rohm and Haas Company.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
The Mouse C3a protein is useful as a standard in a sandwich ELISA to measure mouse C3a protein levels. Purified anti-mouse C3a clone I87-1162 as capture antibody can be paired with the biotinylated I87-419 clone as the detection antibody. The purified antibody should be titrated between 1-4 µg/ml to determine the optimal ELISA plate-coating concentration. For plate coating, we recommend to use a pH 6.5 phosphate buffer.To obtain linear standard curves, doubling dilutions of purified mouse C3a protein ranging from 781 pg/mL to 50 ng/mL are recommended for inclusion in each ELISA plate.This ELISA antibody pair shows no cross-reactivity with the following: recombinant mouse C5a, native human C3a, C4a and C5a, and rat C5a. This ELISA antibody pair shows a limited cross-reactivity with rat C3a.
The amount of protein provided is listed on the vial label, record the batch number and the concentration of the reconstituted standard here and on the vial for future reference: ______ ng/mL.
Warning:Mouse C3a protein (Cat. No. 558618) contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).
Hazard statements
May cause an allergic skin reaction.
Precautionary statements
Wear protective gloves / eye protection.
Wear protective clothing.
Avoid breathing mist/vapours/spray.
If skin irritation or rash occurs: Get medical advice/attention.
IF ON SKIN: Wash with plenty of water.
Dispose of contents/container in accordance with local/regional/national/international regulations.
