Description
PARP [Poly(ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from the 116 kDa intact form into 85 kDa and 25 kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis. BD Pharmingen™ PARP antibodies [Clone 4C10-5 (Cat. No. 556494), Clone 7D3-6 (Cat. No. 556493), and Clone C2-10 (Cat. No. 556362)] all recognize both the intact 116 kDa form and 85 kDa fragment of PARP.
The 7D3-6 antibody recognizes both the 116 kDa intact form and the 85 kDa fragment of human PARP. It recognizes both native and denatured PARP. It reacts with an epitope located in the NAD binding domain. Purified human PARP was used as immunogen. The antibody was originally characterized by western blot analysis, flow cytometric analysis, and by dot blot assays. In dot blot assays, the antibody reacts with the native enzyme in the presence or absence of bound DNA as well as after synthesis of covalently linked poly(ADP-ribose).
Format
- FormatPurified
Suggested Companion Products
Jurkat Cell Lysate RUO 500µgCat No: 611451
Jurkat Apoptotic Lysate Set I RUO 500µgCat No: 550959
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20°C). Additional control lysate is sold separately.
