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当前位置: 首页 > 产品中心 > peptide > BD Biosciences/Anti- R-Phycoerythrin (PE) Magnetic Particles - DM/5mL/557899
商品详细BD Biosciences/Anti- R-Phycoerythrin (PE) Magnetic Particles - DM/5mL/557899
BD Biosciences/Anti- R-Phycoerythrin (PE) Magnetic Particles - DM/5mL/557899
BD Biosciences/Anti- R-Phycoerythrin (PE) Magnetic Particles - DM/5mL/557899
商品编号: 557899
品牌: bdbiosciences
市场价: ¥10395.00
美元价: 5940.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product DetailsRecommended AssayReferences

Description

BD IMag™ Anti- R-Phycoerythrin (PE) Magnetic Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. The E31-1459 antibody clone reacts with PE, a commonly used fluorochrome for flow cytometry. The binding of the E31-1459 antibody to PE has been reported not to quench the fluorescence of the PE molecule. These magnetic particles are optimized for the positive selection or depletion of leukocyte subpopulations using the BD IMag™ Cell Separation Magnet.

Leukocytes are labelled with BD IMag™ Anti- R-Phycoerythrin (PE) Magnetic Particles - DM according to the Magnetic Labeling and Separation Protocol. In brief, cells are labeled with a PE-conjugated antibody that recognizes the subpopulation of interest.After washing away excess antibody, BD IMag™ Anti- R-Phycoerythrin (PE) Magnetic Particles - DM are added to the cell suspension and bind the PE-conjugated antibody on the cells. This labeled cell suspension is then placed within the magnetic field of the Cell Separation Magnet.Positive selection or depletion is then performed.Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (depleted or negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The selections are repeated twice to increase the purity of the positive fraction and the yield of the depleted fraction. The magnetic separation steps are diagrammed in the Depletion and Positive Selection Flow Charts. After the positive fraction has been washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

Suggested Companion Products

Buffer (10X) RUO 100mLCat No: 552362

Cell Separation Magnet RUO Cat No: 552311

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) 2.4G2RUO 0.1mgCat No: 553141

Purified Mouse Anti-Rat CD32 D34-485RUO 0.1mgCat No: 550270

PE Hamster Anti-Mouse CD3e 145-2C11RUO 0.1mgCat No: 553063

PE Rat Anti-CD11b M1/70RUO 0.2mgCat No: 553311

PE Rat Anti-Mouse CD45R/B220 RA3-6B2RUO 0.1mgCat No: 553089

PE Rat Anti-Mouse Ly-6G and Ly-6C RB6-8C5RUO 0.1mgCat No: 553128

PE Mouse Anti-Human TCR γδ B1RUO 0.1mgCat No: 555717

Preparation and Storage

Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. BD IMag™ particles are prepared from carboxy-functionalized magnetic particles which are manufactured by Skold Technology and are licensed under US patent number 7,169,618.
  5. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
  6. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

品牌介绍
BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。BD公司具有强大的研发能力和世界上最棘手的多种疾病进行斗争的能力。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务遍及全球。公司的业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。公司的服务对象包括医疗机构,生命科学研究所,临床实验室,工业单位和普通大众。BD has state-of-the-art facilities around the globe that provide an environment that enables our highly talented workforce to be the best at their professions. We hire associates who have a passion and commitment to advancing the world of health. We are always seeking great people to join our company on its journey to greatness.BDRat Granulocytes Pure RP-1  500ug550002BDMs Ig Kpa Lgt Chain FITC 187.1  500ug550007BDICC Fixation Buffer 100mL550011BDMs CD210 NALE 1B1.3a 500ug550019BDMs CD51 Pure RMV-7 100ug550026BDMs CD5 APC 53-7.3 100ug550037BDHu CD62E PE-Cy5 68-5H11 100Tst550041BDHu CD95 Pure EOS9.1  100ug550052BDHam IgG2 Kpa ItCl FITC B81-3 250ug550057BDMs Crry/p65 Pure 1F2 100ug550059BDMs IL-4 Recom Protein 10ug550068BDMs IL-2 Recom Protein 20ug550070BDHu IL-6 Recom Protein 10ug550072BDHu MIP-1 Bta PE D21-1351 100ug550082BDMs IgG1 PE A85-1 100ug550084BDHam IgG2 Kpa ItCl PE B81-3 100ug550086BDHu CD142 Pure HTF-1 100ug550254BDHu CD1d PE CD1d42 100Tst550256BDHu CD73 PE AD2 100Tst550258BDHu CD103 FITC Ber-ACT8 100Tst550260BDMs CD11c APC HL3 100ug550268BDMs CD72b/c Aloatg PE JY/93 100ug550270BDRat CD32 Pure D34-485 500ug550272BDRat CD32 NALE D34-485 500ug550274BDMs CD3e IHC Pure 145-2C11 1mL550277BDMs CD4 IHC Pure H129.19 1mL550280BDMs CD8a IHC Pure 53-6.7 1mL
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