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当前位置： 首页 > 产品中心 > peptide > BD Biosciences/Purified Mouse Anti-Human CD34/20 μL/348050
商品详细BD Biosciences/Purified Mouse Anti-Human CD34/20 μL/348050
BD Biosciences/Purified Mouse Anti-Human CD34/20 μL/348050
BD Biosciences/Purified Mouse Anti-Human CD34/20 μL/348050
商品编号： 348050
品牌： bdbiosciences
市场价： ¥6005.16
美元价： 3431.52
产地： 美国(厂家直采)
公司：
产品分类： 多肽合成
公司分类： peptide
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍
Product DetailsRecommended AssayReferences

Description

CD34 (Anti–HPCA-1), clone My10, is derived from the hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.

CD34 (Anti–HPCA-2), clone 8G12, is derived from the hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.

The CD34 antigen is a single-chain transmembrane glycoprotein, Mr 105 to 120 kilodaltons (kd). The antigen is associated with human hematopoietic progenitor cells and is a differentiation stage–specific leucocyte antigen. Clone My10 and clone 8G12 recognize two distinct CD34 epitopes; at least three epitopes have been identified.

Format
  • FormatPurified

Other Formats→

  • APC
  • FITC
  • PE
  • PE-Cy™7
  • PerCP
  • PerCP-Cy™5.5
    Resources & Tools
    SpectrumViewerDownload MSDSDownload TDS
    Preparation and Storage

    Clone My10: The monoclonal antibody is supplied as 100 µg purified immunoglobulin in 2.0 mL (50 µg/mL) of phosphate-buffered saline (PBS).

    Clone 8G12: The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of PBS. The FITC and PE conjugates are each supplied as 50 µg in 2.0 mL (25 µg/mL) of PBS. The PerCP conjugate is supplied as 50 µg in 1.0 mL (50 µg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 12.5 µg in 1.0 mL (12.5 µg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 µg in 0.5 mL (50 µg/mL) of PBS. The APC conjugate is supplied as 50 µg in 0.5 mL (100 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

    1. Andrews RG, Singer JW, Bernstein ID. Precursors of colony-forming cells in humans can be distinguished from colony-forming cells by expression of the CD33 and CD34 antigens and light scattering properties. J Exp Med. 1989;169:1721-1731.

    2. Brocklebank AM, Sparrow RL. Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry. 2001;46:254-261.

    3. Civin C, Banquerigo M, Strauss L, Loken MR. Antigenic analysis of hematopoiesis. VI. Characterization of MY-10-positive progenitor cells in normal human bone marrow. Exp Hematol. 1987;15:10-17.

    4. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis. III. A hemopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 1984;133:157-165.

    5. Civin CI, Trischmann TM, Fackler MJ, et al. Report on the CD34 cluster workshop. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:818-825.

    6. Gore SD, Kastan MB, Civin CI. Normal human bone marrow precursors that express terminal deoxynucleotidyl transferase include T-cell precursors and possible lymphoid stem cells. Blood. 1991;77:1681-1690.

    7. Greaves MF, Titley I, Colman SM, et al. CD34 cluster workshop report. In: Schlossman SF, Boumsell L, Gilks W, et al, eds. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Osford University Press; 1995;1:840-846.

    8. Hurwitz CA, Loken MR, Graham ML, et al. Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. Blood. 1988;72:299-307.

    9. Kurtzberg J, Denning SM, Nycum LM, Singer KH, Haynes BF. Immature human thymocytes can be driven to differentiate into nonlymphoid lineages by cytokines from thymic epithelial cells. Proc Natl Acad Sci USA. 1989;86:7575-7579.

    10. Lansdorp P, Sutherland H, Eaves C. Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow. J Exp Med. 1990;172:363-366.

    11. Lansdorp PM, Dougherty GJ, Humphries RK. CD34 epitopes. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:826-827.

    12. Leary AG, Strauss LC, Civin CI, Ogawa M. Disparate differentiation in hemopoietic colonies derived from human paired progenitors. Blood. 1985;6:327-332.

    13. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. I. Normal erythroid development. Blood. 1987;69:255-263.

    14. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B-lymphocyte development. Blood. 1987;70:1316-1324.

    15. Lu L, Walker D, Broxmeyer H, Hoffman R, Hu W, Walker E. Characterization of adult human marrow hematopoietic progenitors is highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies. J Immunol. 1987;139:1823-1829.

    16. Peschel C, Köller U. Cluster report: CD34. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:817-818.

    17. Ryan D, Kossover S, Mitchell S, Frantz C, Hennessy L, Cohen H. Subpopulations of common acute lymphoblastic leukemia antigen-positive lymphoid cells in normal bone marrow identified by hematopoietic differentiation antigens. Blood. 1986;68:417-425.

    18. Siena S, Bregni M, Brando B, et al. Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. Blood. 1991;77:400-409

    19. Terstappen LWMM, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood. 1991;77:1218-1227.

    20. Terstappen LWMM, Safford M, Könemann S, et al. Flow cytometric characterization of acute myeloid leukemia. Part II. Phenotypic heterogeneity at diagnosis. Leukemia. 1992;6:70-80.

    品牌介绍
    BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗，提高传染性疾病诊断的质量和速度，推进新型药物和疫苗的研究与发现。BD公司具有强大的研发能力和世界上最棘手的多种疾病进行斗争的能力。公司于1897年在纽约成立，总部位于美国新泽西州的富兰克林湖，业务遍及全球。公司的业务可分为BD医疗、BD诊断、BD生物科学三大类，生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。公司的服务对象包括医疗机构，生命科学研究所，临床实验室，工业单位和普通大众。BD has state-of-the-art facilities around the globe that provide an environment that enables our highly talented workforce to be the best at their professions. We hire associates who have a passion and commitment to advancing the world of health. We are always seeking great people to join our company on its journey to greatness.BDRat Granulocytes Pure RP-1  500ug550002BDMs Ig Kpa Lgt Chain FITC 187.1  500ug550007BDICC Fixation Buffer 100mL550011BDMs CD210 NALE 1B1.3a 500ug550019BDMs CD51 Pure RMV-7 100ug550026BDMs CD5 APC 53-7.3 100ug550037BDHu CD62E PE-Cy5 68-5H11 100Tst550041BDHu CD95 Pure EOS9.1  100ug550052BDHam IgG2 Kpa ItCl FITC B81-3 250ug550057BDMs Crry/p65 Pure 1F2 100ug550059BDMs IL-4 Recom Protein 10ug550068BDMs IL-2 Recom Protein 20ug550070BDHu IL-6 Recom Protein 10ug550072BDHu MIP-1 Bta PE D21-1351 100ug550082BDMs IgG1 PE A85-1 100ug550084BDHam IgG2 Kpa ItCl PE B81-3 100ug550086BDHu CD142 Pure HTF-1 100ug550254BDHu CD1d PE CD1d42 100Tst550256BDHu CD73 PE AD2 100Tst550258BDHu CD103 FITC Ber-ACT8 100Tst550260BDMs CD11c APC HL3 100ug550268BDMs CD72b/c Aloatg PE JY/93 100ug550270BDRat CD32 Pure D34-485 500ug550272BDRat CD32 NALE D34-485 500ug550274BDMs CD3e IHC Pure 145-2C11 1mL550277BDMs CD4 IHC Pure H129.19 1mL550280BDMs CD8a IHC Pure 53-6.7 1mL
    公司介绍
    BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗，提高传染性疾病诊断的质量和速度，推进新型药物和疫苗的研究与发现。BD公司具有强大的研发能力和世界上最棘手的多种疾病进行斗争的能力。公司于1897年在纽约成立，总部位于美国新泽西州的富兰克林湖，业务遍及全球。公司的业务可分为BD医疗、BD诊断、BD生物科学三大类，生产销售包
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