Description
Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-VAD-FMK is a cell permeable general caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and inhibits apoptosis. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. Z-VAD-FMK has a molecular weight of 467 Daltons.
Format
- FormatPurified
Suggested Companion Products
PE Annexin V Apoptosis Detection Kit I RUO Cat No: 559763
Z-FA-FMK, Negative Control for Caspase Inhibitors RUO 1mgCat No: 550411
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Preparation and Storage
Avoid multiple freeze-thaws of product.Store the lyophilized Z-VAD-FMK inhibitor at -20˚C. Reconstitute the Z-VAD-FMK inhibitor in DMSO before use. The reconstituted Z-VAD-FMK inhibitor may be stored in small aliquots at -20˚C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
The Z-VAD-FMK inhibitor is designed to be used in both in vivo and in vitro cell based assays to measure the inhibition of apoptosis. Reconstitute 1.0 mg of the Z-VAD-FMK inhibitor in DMSO. A 10 mM stock solution may be made by dissolving 1.0 mg of Z-VAD-FMK in 214 µl DMSO. The final concentration of inhibitor may vary between experimental systems and investigators are encouraged to titrate the inhibitor for optimal performance. As a precautionary note, do not exceed a final DMSO concentration of 0.2% as higher levels may cause cellular toxicity and mask the effects of the caspase inhibitor.
