Description
The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Q-VD-OPh is a potent, cell-permeable inhibitor of caspase activity. It irreversibly inhibits the activity of a broad range of caspases, including caspases-1, -2, -3, -5, -6, -7, -8, -9, -10, and -12. Deemed a next generation caspase inhibitor, Q-VD-OPh has increased selectivity for the caspases over other cysteine proteases, increased potency, and decreased toxicity compared to the previous generation of caspase inhibitors. Additionally, other pan caspase inhibitors do not inhibit all caspases equally. Q-VD-OPh, in contrast, inhibits the spectrum of caspases more equally and at lower concentrations, thus making it a true broad spectrum caspase inhibitor.
Suggested Companion Products
PE Annexin V RUO 200TestsCat No: 556421
7-AAD RUO 2mLCat No: 559925
PE Annexin V Apoptosis Detection Kit I RUO Cat No: 559763
Z-FA-FMK, Negative Control for Caspase Inhibitors RUO 1mgCat No: 550411
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Preparation and Storage
Avoid multiple freeze-thaws of product.Store the lyophilized Q-VD-OPh inhibitor at –20°C. Reconstitute the Q-VD-OPh inhibitor in DMSO before use. The reconstituted Q-VD-OPh inhibitor may be stored in small aliquots at –20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
The Q-VD-OPh inhibitor is designed to be used in both in vivo and in vitro cell based assays to measure the inhibition of apoptosis.
Reconstitute 1.0 mg of the Q-VD-OPh inhibitor in DMSO. A 10 mM stock solution may be made by dissolving 1.0 mg of Q-VD-OPh in 195 µl DMSO. The final concentration of inhibitor may vary between experimental systems and investigators are encouraged to titrate the inhibitor for optimal performance. As a precautionary note, do not exceed a final DMSO concentration of 0.2% as higher levels may cause cellular toxicity and mask the effects of the caspase inhibitor.
