Description
This product contains a mixture of 3.0 - 3.4 µm Rainbow Calibration Particles that are dyed to six different fluorescent intensities.Rainbow Calibration Particles (approx. 1 x 10^7 particles/mL) are designed for routine calibration of flow cytometers and contain a mixture of fluorophores that are excited at any wavelength from 365 - 650 nm.Rainbow Calibration Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.Before use, mix or resuspend the particles by vortexing.Dilution of 3 - 5 drops of particles to 1 mL of sheath fluid will provide an adequate number of particles for flow cytometric analysis.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.
Product Notices
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.