Description
The vial contains a mixture of 2.5 - 4.5 µm Nile Red Fluorescent Particles that are dyed to have an excitation maximum of approximately 525 nm with a subsequent emission maximum of approximately 560 nm. This particle mixture is useful with flow cytometers equipped with a 488 nm argon laser. This particle mixture has compatible excitation and emission spectra with R-Phycoerythrin and Propidium Iodide.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This particle mixture (~1.0% w/v) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric anaylsis.
