Description
The vial contains a single population of Blank Calibration Particles that are dyed to a single fluorescent intensity. The intensity of this particle corresponds to the dimmest intensity. The intensity of this particle corresponds to the dimmest intensity peak in the Rainbow Calibration Particles, 6.0 - 6.4 µm (Cat. No. 5556288). Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from365 - 650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.
| Resources & Tools | ||||||
|---|---|---|---|---|---|---|
| SpectrumViewer | Download TDS | Regulatory Document Website | ||||
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This particle mixture (~10x10^6 particles/ml) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.
