Description
CD1a, clone SK9, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from CD-1 mice immunized with human thymocytes.
CD1a recognizes a human thymocyte antigen, Mr 49 kilodaltons (kd), associated with 12-kd β2 microglobulin.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 25 μg in 1.0 mL (25 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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