Description
CD11b, clone D12, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood T lymphocytes.
CD11b recognizes a human leucocyte antigen that is the C3bi complement receptor (CR3). CD11b is specific for the Mr 165-kilodalton (kd) α subunit of the CD11b/CD18 antigen heterodimer. The Mr 95-kd CD18 antigen, the β chain of leucocyte function–associated antigen-1 (LFA-1β), is common to the CD11a/CD18 (LFA-1) and CD11c/CD18 antigen heterodimers.
Format
- FormatAPC
- Excitation SourceRed 633 nm
- Excitation Max650nm
- Emission Max660nm
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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Preparation and Storage
The monoclonal antibody is supplied as 100 µg purified immunoglobulin in 2.0 mL (50 µg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 100 µg in 2.0 mL (50 µg/mL). The APC conjugate is supplied as 25 µg in 0.5 mL (50 µg/mL). PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
